Table 1.
List of miRNAs with functional role in endometriosis and/or nociception. TargetScan and IPA software analysis were used to identify target genes with functional role in inflammatory/nociceptive, epigenetic and endometriosis. Bolded genes were further investigated in this study. Fold change values were based on the Qiagen MiRNome qPCR array and associated SA Biosciences software. RT-qPCR analysis showed that compared to control tissues, the mRNA expression of BCL2 (fold change=0.75), DNMT3B (fold change=0.57), and OPRM1 (fold change=0.51) were lower in endometriotic tissues, as indicated by ↓. For all tissue miRNome array data, p<0.05.
| miRNA | Fold change (Tissues, array) | GENE (pain/inflamm) | GENE (epigenetic) | GENE (endometriosis) |
|---|---|---|---|---|
| hsa-miR-29a | 6.46 | CNR1, CX3CL1 | KDM5A/5 C/6B/4B, PHF21A, DNMT3A, DNMT3B ↓↓ | BCL2 ↓ |
| hsa-miR-148a | 4.41 | PTGES3 | SIRT1, COX1, KDM6B | DNMT1, DNMT3B ↓↓ |
| hsa-miR-100 | 4.10 | mTOR | mTOR, IG1R | |
| hsa-miR-548l | 0.09 | OPRM1 ↓↓ | ||
| hsa-let-7g | 3.45 | NGF, OPRM1 ↓↓, SCN11A, IL6 |