Figure 5.

Engineering and properties of soluble NKG2D multimer. (A) Ribbon representations of the crystal structures (colored by chain) of the C4bbp heptamerization motif (top) and the NKG2D homodimeric ectodomain recognition unit (bottom). Disulfide bonds are shown as gray-and-yellow spheres. (B and C) Schematic representations of the native NKG2D homodimer and the NKG2D^scd₇ protomer, detailing key features. (D) Schematic representation of the overall molecular arrangement of the NKG2D^scd₇ multimer. (E) Comparative reduced/nonreduced polyacrylamide gel electrophoresis analysis confirming purity and disulfide bond formation. (F) Analytical SEC showing solution monodispersivity of human NKG2D^scd₇ multimer.