Fig. 3.

A549 and BEAS-2B cells were treated with 1 µM B[a]P and incubated at the indicated pH_e for 6, 24 and 48 h. The left column represents data from A549 cells and the right column from BEAS-2B cells. The extracellular concentrations of B[a]P-9,10-diol (a, b); B[a]P-7,8-diol (c, d); B[a]P-3-OH (e, f); and B[a]P (g, h) were measured at the indicated time points. Data are presented as mean ± SEM, n = 4 independent experiment. The results of statistical analysis are included in supplemental Table 1