Table 1.
Cell viability test based on MTT assay and trypan blue after incubated A549 cells and BEAS-2B cells for 6, 24 and 48 h under combination of both 1 μM B[a]P with different pH (pH 7.8, 7, 6.5, 6 and 5.5) mediums
| Methods | Time (h) | A549 cells | BEAS-2B cells | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pH 7.8 | pH 7 | pH 6.5 | pH 6 | pH 5.5 | pH 7.8 | pH 7 | pH 6.5 | pH 6 | pH 5.5 | ||
| MTT assay | 6 | 100 ± 1.2 | 92.1 ± 5.7 | 91.0 ± 3.9 | 93.4 ± 4.8 | 95.6 ± 5.2 | 100 ± 8.6 | 80.4 ± 7.8** | 79.3 ± 5.5** | 66.9 ± 3.2*** | 55.5 ± 3.8*** |
| 24 | 100 ± 5.3 | 90.7 ± 8.9 | 95.5 ± 4.6 | 88.3 ± 3.6 | 87.7 ± 5.4 | 100 ± 17.2 | 56.6 ± 14.1*** | 53.3 ± 6.5*** | 40.8 ± 9.2*** | 31.5 ± 5.9*** | |
| 48 | 100 ± 5.1 | 83.5 ± 8.2 | 98.9 ± 1.2 | 95.5 ± 3.8 | 95.1 ± 3.3 | 100 ± 6.2 | 71.3 ± 1.9*** | 63.5 ± 6.8*** | 55.1 ± 7.1*** | 4.5 ± 0.3*** | |
| Trypan blue | 6 | 98.0 ± 1.2 | 95.6 ± 3.0 | 95.1 ± 1.4 | 96.5 ± 0.8 | 93.7 ± 2.8 | 95 ± 0.1 | 97 ± 0.1 | 98 ± 0.1 | 96 ± 0.1 | 94.3 ± 1.2 |
| 24 | 98.3 ± 1.2 | 97.7 ± 0.1 | 97.9 ± 1.5 | 95.5 ± 3.9 | 94.1 ± 1.0 | 96 ± 2 | 96.3 ± 1.5 | 92.3 ± 1.5* | 59.7 ± 1.2*** | 54.7 ± 3.2*** | |
| 48 | 98.9 ± 0.7 | 97.7 ± 1.9 | 96.2 ± 2.8 | 94.4 ± 3.8 | 98.4 ± 1.3 | 96.3 ± 0.6 | 94.7 ± 1.2 | 93.3 ± 1.5* | 65.3 ± 0.6*** | 34 ± 3*** | |
Results of MTT are expressed as percentage of pH 7.8. Results of trypan blue are presented as survival rate (total live cells/total cells). All the value is presented as mean % ± SEM, n = 6
* p < 0.05
** p < 0.01
*** p < 0.001