Fig. 2.

Colony PCR of transformed E. coli. CBSV and UCBSV specific primers were used to amplify the appropriate cloned coat protein fragments. Primers used are indicated on the top of the gel picture. A – represents bacteria colony, B – represents corresponding purified plasmids of the bacteria colony 1 and 2, the numbers 1–3 = bacterial colony, 4–5 = purified plasmid, 6 = positive control, L = 1 Kb DNA ladder, −C = negative control (PCR water).