Fig. 1.

Systemic exposure to tartrazine results in a periportal inflammatory cell recruitment in mice. Adult male C57Bl/6 mice were administered either tartrazine (T, 4 animals), E2 (4 animals) or relevant vehicle control (PBS for tratrazine, 3 animals; 1:19 ethanol:olive oil (v/v) for E2, 4 animals) by 10 daily intraperitoneal injections over 14 days. A, serum ALP, *significantly different activity versus vehicle control using the Student’s t-test (two tailed), p > 0.95. B, H&E-stained liver sections from animals treated as indicated, typical views chosen. C, quantification of portal tract inflammatory cells, *significantly different number of portal tract cells versus vehicle control using the Student’s t-test (two tailed), p > 0.95 based on at least 10 randomly selected portal tracts per animal. D, sirius red-stained liver sections from animals treated as indicated, typical views chosen. E, quantification of sirius red positive stained area, *significantly different activity versus vehicle control using the Student’s t-test (two tailed), p > 0.95.