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. 2017 May;42(3):275–283.

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Copyright: © Iranian Journal of Medical Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression and purification of L-alanine dehydrogenase (AlaDH) antigen samples, (lanes 1–5) resolved in a 12% (w/v) polyacrylamide gel, under reducing conditions followed by Coomassie blue staining. Escherichia coli (E. coli) extracts before isopropyl D-thiogalacto-pyranoside (IPTG) induction (lane 1). E. coli extracts 4 hours after induction with 0.1 mM of IPTG (lane 2). Washed Ni-NTA resin (lanes 3 and 4). Recombinant proteins purified by affinity chromatography using Ni-NTA resin with thrombin cleavage (lane 5). Molecular weight standards are shown to the left of lane 1.