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. 2017 May 12;12:73. doi: 10.1186/s13018-017-0572-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Immunohistochemical staining of PGP 9.5 at 6 months after surgery. The intact AF showed sparsely PGP 9.5-positive nerves distributed within the outermost layers of the AF (a). The punctured disc exhibited positive staining for PGP 9.5 scattered in superficial areas of the scar tissue (b). Small nerves were detected in the deeper outer AF along the fissures toward the NP (c). Nerves were seen deep within the inner AF, in close proximity to blood vessels (d)