Table 1.
List of primers and gene markers used in this study as MLST scheme
| Target gene | Enzyme entry | Chromosomal location | Gene length (bp) | Amplicon size (bp) | Primer sequence | Reference |
|---|---|---|---|---|---|---|
| Glucose-6-phosphate dehydrogenase (g6pdh) | EC 1.1.1.49 | 20 and 34aa | 1,686 | 881 | Fw: ATGGAAGCGTGTGATCGAAT Rv: GGCTCAACACACTTCAGCAA |
[16] |
| Phosphoglucomutase (pgm) | EC 5.4.2.2 | 21 | 1,770 | 529 | Fw: CAGAGAAGCTGACGTCCCAG Rv: GACGGGTTCACGAAGAAGCG |
[21] |
| Mannose phosphate isomerase (mpi) | EC 5.3.1.8 | 32 | 1,287 | 681 | Fw: GGCAAGATGTATGCGGAGTT Rv: CTCCCCAGGAACCATCTGTA |
[16] |
| Alanine aminotransferase (alat) | EC 2.6.1.21 | 12 | 1,494 | 589 | Fw: GTGTGCATCAACCCMGGGAA Rv: CGTTCAGCTCCTCGTTCCGC |
[21] |
| Aspartate aminotransferase (asat) | EC 2.6.1.1 | 24 | 1,169 | 684 | Fw: ACGAGCGCCGTCCGYAA Rv: TTCCYMCATCCACCAAGC |
[15] |
| Isocitrate dehydrogenase (icd) | EC 1.1.1.42 | 33 | 1,278 | 1,022 | Fw: GAATCGGGAAGGAGATCACA Rv: CATCATAGCCCCAGAGAGGA |
[16] |
| Cytochrome b (cytb) | Maxicircle | 618 | Fw: AGCGGAGAGRARAGAAAAGG Rv: GYTCRCAATAAAATGCAAATC |
a g6pdh gene is present on chromosomes 20 and 34a only in L. (V) braziliensis; it is present on chromosome 20 in L. (V) panamensis uniquely