Fig. 6.

Motifs differentially enriched in early- and late-methylating CpG islands. a Summary of results of DREME analysis identifying motifs differentially enriched in CGIs that become fully methylated in GV oocytes grouped according to methylation level in 60–65 µm oocytes. Codes 0–25, 25–50, 50–75 and 75–100 represent CGIs with corresponding percentage methylation in 60–65 µm oocytes. The numbers of CGIs in each category are 470, 329, 384 and 63, respectively. b DREME motifs significantly enriched in CGIs methylated ≤25% in oocytes compared with ≥75% methylated CGIs that correspond to binding site motifs for known TFs. In motif sequence, B = C/G/T and M = C/A. P value and E values are as defined by DREME and binding sites as identified by Tomtom