Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
qPCR data quality summary
| Data quality metric | 2015 | 2016 | ||
| Accuracy in composite standard curves | ||||
| Number of standard curves | 9 | 9 | ||
| R2 | 0.996 | 0.999 | ||
| Amplification efficiency | 1.933 | 2.097 | ||
| Precision: calibrator samples CV | ||||
| Number of samples | 55 | 71 | ||
| Enterococci target CV | 2.05% | 1.33% | ||
| SPC target CV | 1.11% | 1.41% | ||
| Inhibition | Frequency | Percent | Frequency | Percent |
| ΔCtSPC < 2 | 536 | 99.26 | 1210 | 96.3% |
| 2 ≤ ΔCtSPC < 3 | 2 | 0.37% | 32 | 2.5% |
| ΔCtSPC ≥ 3 | 2 | 0.37% | 14 | 1.1% |
| Total | 540 | 100% | 1258 | 100% |
CV coefficient of variation
