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. 1979 Jun;63(6):989–994. doi: 10.1104/pp.63.6.989

Factors Affecting Crown Gall Tumorigenesis in Tuber Slices of Jerusalem Artichoke (Helianthus tuberosus, L.) 1

Eugene Tanimoto a, Carl Douglas a, Walter Halperin a,2
PMCID: PMC542958  PMID: 16660891

Abstract

Agrobacterium tumefaciens can induce tumors on thin slices which are excised from Jerusalem artichoke (Helianthus tuberosus) tubers and grown in culture on medium containing minerals and a carbon source. A comparative study was made of the kinetics of cell division in slices under three conditions: (a) slices which were untreated and showed only spontaneous (wound-induced) cell divisions; (b) slices treated with indoleacetic acid at several concentrations; and (c) slices treated with virulent or avirulent bacteria. The earliest spontaneous cell divisions were completed (as detected by the appearance of new daughter cell pairs) by about 3 hours. These cells divide only once. In indoleacetic acid-treated tissue, more cells divide, with the first cell pairs being detected slightly earlier than in slices not subjected to the hormone. The number of cells which divide is roughly proportional to auxin concentration. Tissue treated with virulent bacteria showed only the pattern of spontaneous cell division until about 72 hours, after which another burst of cell division commenced and continued indefinitely. The bacteria-induced growths produced the unusual amino acids which are characteristic of crown gall tumors. The percentage of slices with tumors was sharply reduced if certain avirulent A. tumefaciens strains were applied prior to virulent strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun A. C. A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell. Proc Natl Acad Sci U S A. 1958 Apr;44(4):344–349. doi: 10.1073/pnas.44.4.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chilton M. D., Drummond M. H., Merio D. J., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977 Jun;11(2):263–271. doi: 10.1016/0092-8674(77)90043-5. [DOI] [PubMed] [Google Scholar]
  3. Glogowski W., Galsky A. G. Agrobacterium tumefaciens Site Attachment as a Necessary Prerequisite for Crown Gall Tumor Formation on Potato Discs. Plant Physiol. 1978 Jun;61(6):1031–1033. doi: 10.1104/pp.61.6.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KAPER J. M., VELDSTRA H. On the metabolism of tryptophan by Agrobacterium tumefaciens. Biochim Biophys Acta. 1958 Nov;30(2):401–420. doi: 10.1016/0006-3002(58)90065-9. [DOI] [PubMed] [Google Scholar]
  5. Lippincott B. B., Lippincott J. A. Bacterial attachment to a specific wound site as an essential stage in tumor initiation by Agrobacterium tumefaciens. J Bacteriol. 1969 Feb;97(2):620–628. doi: 10.1128/jb.97.2.620-628.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lippincott B. B., Whatley M. H., Lippincott J. A. Tumor induction by agrobacterium involves attachment of the bacterium to a site on the host plant cell wall. Plant Physiol. 1977 Mar;59(3):388–390. doi: 10.1104/pp.59.3.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lippincott J. A., Lippincott B. B. The genus Agrobacterium and plant tumorigenesis. Annu Rev Microbiol. 1975;29:377–405. doi: 10.1146/annurev.mi.29.100175.002113. [DOI] [PubMed] [Google Scholar]
  8. Van Larebeke N., Engler G., Holsters M., Van den Elsacker S., Zaenen I., Schilperoort R. A., Schell J. Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature. 1974 Nov 8;252(5479):169–170. doi: 10.1038/252169a0. [DOI] [PubMed] [Google Scholar]
  9. Watson B., Currier T. C., Gordon M. P., Chilton M. D., Nester E. W. Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1975 Jul;123(1):255–264. doi: 10.1128/jb.123.1.255-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Yamada S., Itano H. Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines. Biochim Biophys Acta. 1966 Dec 28;130(2):538–540. doi: 10.1016/0304-4165(66)90256-x. [DOI] [PubMed] [Google Scholar]

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