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. 2017 Apr 4;7:595. doi: 10.1038/s41598-017-00734-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IR of p27KO cells is associated to mitotic aberrations. (a) Graph reports the quantification of aberrant mitoses encountered in the different cell clones when non-irradiated (NIR, left panels) or irradiated with 2 Gy (right panels) at 48 hours after radiation. Data are expressed as % of total cells. At the bottom, the aberrations were sub-classified respect to mitotic phases. (b) Representative pictures of cells reporting the above mentioned mitotic aberrations. Cells were stained for phospho-Ser10 Histon-H3 (pS10-H3) to identify mitotic cells (pseudocolored in green, shown in white), αTubulin to highlight the mitotic spindle (pseudocolored in red, shown in white) and DAPI to identify the cell nuclei (pseudocolored in blue, shown in white). Only the merged picture is shown with colors.