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. 2017 Apr 6;7:693. doi: 10.1038/s41598-017-00782-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of IL-1β, IL-6 and COX-2 by SAA1 in human amnion fibroblasts. (A–D) Effect of SAA1 (5, 10 and 50 ng/mL, 24 hours) on the amounts of IL-1β, IL-6 mRNA (A, n = 4), COX-2 mRNA and protein (B) n = 4), IL-1β and IL-6 secretion (C, n = 5), and PGE2 secretion (D, n = 3) in human amnion fibroblasts. Top panel of B is a representative immunoblot. Data are the means ± SEM. Statistical analysis was performed with one-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control (0).