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. 2017 Apr 6;7:672. doi: 10.1038/s41598-017-00796-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SOD2 overexpression ameliorated NaF-induced oxidative injury in TCMK-1 cells. TCMK-1 cells were transfected with SOD2 expression construct, then, cells were treated with or without NaF of 3 mM for an additional 12 h. (a) The representative immunoblot and quantification analysis of SOD2. (b) SOD2 activity was measured based on an enzymatic reaction using a SOD2 assay kit. (c) Cell viability was measured. (d) Mitochondrial-derived O₂ ^•− production. (e) 8-OHdG levels. (f) Reduced GSH content. All results are representative of three independent experiments and values are presented as means ± s.d. ^**p < 0.01 versus scramble group, ^##p < 0.01 versus the NaF + scramble group. Full-length blots/gels are presented in Supplementary Figure S12.