Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 6;7:672. doi: 10.1038/s41598-017-00796-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PGC-1α interacts with NRF2 to mediates SIRT3 expression. (a) SIRT3 mRNA level was measured by qPCR in TCMK-1 cells treated with 3 mM for different times. Data are presented as means ± s.d. of at least three independent experiments. ^*p < 0.05, ^**p < 0.01 versus 0 h. The mRNA and protein expression of SIRT3 were measured with PGC-1α and NRF2 knockdown (b,c) or overexpression (d,e), respectively. Data are presented as means ± SD of at least three independent experiments. ^*p < 0.05, ^**p < 0.01. (f) SIRT3 was knocked down by SIRT3 siRNA transfection, and then the expression of PGC-1α and NRF2 were determined by western blot. (g) The expression of PGC-1α and NRF2 were determined in TCMK-1 cells treated with 3 mM for different times. ^*p < 0.05, ^**p < 0.01 versus 0 h. ChIP-qPCR was performed with NRF2 (h) and PGC-1α (i) antibodies in TCMK-1 cells under excessive NaF exposure. Data are presented as means ± s.d. of at least three independent experiments. ^*p < 0.05, ^**p < 0.01. Full-length blots/gels are presented in Supplementary Figures S18–20.