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. 2017 Apr 6;7:708. doi: 10.1038/s41598-017-00739-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A point mutation in the 5′ UTR of TAOK2 stabilises the RNA G4 and affect its regulatory function. (A) Schematic representation of three different mRNAs of firefly luciferase reporter constructs. Wild-type construct contains full-length 5′ UTR of TAOK2. In the G4-mutated construct, an adenine is replaced by a guanine. To make the G4-deleted construct, 27 nucleotides that predicted to form the G4 were deleted. Schematic drawing of wild-type and mutated G4s are proposed based on RNAfold and QGRS-mapper predictions and in vitro results. (B) CD spectra of TAOK2 wild-type and mutated RNA G4 oligos in KCl. (C) CD melting curves of TAOK2 wild-type and mutated RNA G4 oligos at 260 nm. (D) Relative in vitro translation efficiency of the wild-type, G4-mutated and G4-deleted constructs as judged by measuring firefly luciferase enzymatic activity. Data were analysed using one-way-ANOVA (n ≥ 3, error bars represent the SEM, asterisks (*) indicate significance calculated by the Tukey multiple comparisons test).