Table 1.

Melting temperatures of RNA G4 oligos annealed in 100 mM KCl, 20 mM Tris-HCl, pH 7.5.

RNA G4 oligos		T m (°C)a
BCL2	wt	78 ± 2.1
	mut	64 ± 0.2
Long BCL2 b	wt	74.5 ± 3.5
	mut	58 ± 2
CXCL14	wt	>90c
	mut	73 ± 0.5
TAOK2	wt	68 ± 0.5
	mut	75 ± 1

^a T _m values are expressed as mean ± SD (n ≥ 2).

^bLong BCL2 consists of 22 nucleotides predicted to form the RNA G4 and 20 extra nucleotides from each side.

^c CXCL14 wt RNA G4 oligo could not be completely melted even at 95 °C.