Table 2.

Thermodynamics of the several interactions tested by ITC.

Cohesin	Dockerin	Ka M−1	ΔGo kcal mol−1	ΔH kcal mol−1	TΔSo kcal mol−1	N
CohScaA	Doc1bWT	2.67E7 ± 3.78E6	−10.37	−61.19 ± 0.50	−50.82	1
	Doc1aWT	5.03E8 ± 2.36E8	−12.28	−38.92 ± 0.33	−26.64	1
CohScaB3 WT	Doc1b WT	1.03E7 ± 7.63E5	−9.80	−64.94 ± 0.44	−55.13	1
	Doc1aWT	1.18E8 ± 2.00E7	−11, 23	−50.68 ± 0.29	−39.44	1
	Doc1a I39A	3.86E6 ± 7.66E4	−9.18	−57.26 ± 0.12	−48.07	1
	Doc1a S40A	1.09E8 ± 1.17E7	−11.47	−46.60 ± 0.15	−35.12	1
	Doc1a V43A	1.91E6 ± 3.02E4	−8.94	−52.08 ± 0.14	−43.14	1
	Doc1a Q47A	1.71E7 ± 6.89E5	−10.05	−48.27 ± 0.12	−38.21	1
	Doc1a K54A	1.96E7 ± 1.28E6	−10.42	−59.73 ± 0.25	−49.30	1
	Doc1a Q83A	1.54E8 ± 1.03E7	−11.71	−39.45 ± 0.83	−27.73	1
	Doc1a L87A	2.81E7 ± 1.61E6	−10.53	−59.84 ± 0.19	−49.30	1
	Doc1a I39A + V43A	Nb*	Nb*	Nb*	Nb*	Nb*
	Doc1a V43A + Q47A	4.36E5 ± 9.66E3	−7.81	−48.79 ± 0.39	−40.98	1
CohScaB3 A38Q	Doc1aWT	Nb*	Nb*	Nb*	Nb*	Nb*
CohScaB3 N68A		1.10E8 ± 1.20E7	−11.48	−58.63 ± 0.22	−47.15	1
CohScaB3 N75A		9.09E7 ± 8.96E6	−11.10	−52.70 ± 0.17	−41.60	1
CohScaB3 K77A		4.23E8 ± 7.13E7	−12.12	−57.42 ± 0.23	−45, 30	1
CohScaB3 L79A		7.59E6 ± 3.73E5	−9.71	−51.93 ± 0.20	−42.21	1
CohScaB3 E84A		3.62E7 ± 3.45E6	−10.77	−55.45 ± 0.26	−44.68	1
CohScaB3 H121A		2.66E7 ± 1.74E6	−10.41	−52.63 ± 0.18	−42.27	1
CohScaB3 N124A		5.54E7 ± 4.68E6	−10.89	−52.80 ± 0.19	−41.90	1
CohScaB3 E84A + H121A		1.65E6 ± 2.50E5	−8.62	−66.86 ± 1.49	−58.24	1
CohScaB3 N75A + H121A		2.49E6 ± 6.66E4	−8.93	−50.84 ± 0.18	−41.90	1
CohScaB3 N75A + N124A		1.86E6 ± 3.59E5	−8.85	−56.31 ± 1.56	−47.45	1
CohScaB3 N75A + E84A		2.08E7 ± 1.29E6	−10.47	−51.76 ± 0.18	−41.29	1
CohScaB3 E84A + H121A		1.65E6 ± 2.50E5	−8.62	−66.86 ± 1.49	−58.24	1
CohScaB3 E84A + N124A		1.53E7 ± 9.83E5	−10.05	−55.04 ± 0.23	−44.99	1
CohScaB3 H121A + N124A		2.28E6 ± 9.39E4	−8.84	−46.44 ± 0.24	−37.59	1

All Thermodynamic parameters were determined at 308 K.

*Nb - No binding,