Table 2.

Primers and oligos used for qRT-PCR assay, psi-check2 and lentiCRISPR v2 cloning.

Primer	Sequence	Length (bp)
LHX6-F	5′-CGGAACAGCTGCAGGTTATG-3′	20
LHX6-R	5′-CTGAACGGGGTGTAGTGGAT-3′	20
β-actin-F	5′-CATCCGCAAAGACCTGTACG-3′	20
β-actin-R	5′-CATCCGCAAAGACCTGTACG-3′	20
LHX6-3′ UTR-Mut-F1	5′-TTTCCCTCTCGGACGAGAGAACCAGAAC-CCACCAGGAGCACC-3′	41
LHX6-3′ UTR-Mut-R1	5′-GGCCAGGCCTCGGGAACC-3′	18
LHX6-3′ UTR-Mut-F2	5′-AGTCCCCTTCgacgaGTGGTTAACCTGTTA-TGTTG-3′	35
LHX6-3′ UTR-Mut-R2	5′-TGGGCTAAAACATGCTGATTAG-3′	22
LHX6-sg1-F	5′-CACCGCGTTCTCGGCGGCCCTCTTG-3′	25
LHX6-sg1-R	5′-AAACCAAGAGGGCCGCCGAGAACGC-3′	25
LHX6-sg2-F	5′-CACCGGAAGGACGTCCGCGCGCGCT-3′	25
LHX6-sg2-R	5′-AAACAGCGCGCGCGGACGTCCTTCC-3′	25
miR-214-sg-F	5′-CACCGCATCCGCTCACCTGTACAGC-3′	25
miR-214-sg-R	5′-AAACGCTGTACAGGTGAGCGGATGC-3′	25
AAV-sg-F	5′-CACCGGGGCCACTAGGGACAGGAT-3′	24
AAV-sg-R	5′-AAACATCCTGTCCCTAGTGGCCCC-3′	24