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. 2017 Apr 10;7:777. doi: 10.1038/s41598-017-00914-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Enhancement of the oxygen supply and CEC proliferation in the perfusion system. (A) Photograph of the perfusion system in a 37 °C incubator containing 5% CO₂. (B) Photographic illustration of the perfusion system. (C) Schematic of the sensor system. (D) Photograph of the microsensor. (E) Intensity values of the microsensor for dissolved oxygen. (F) Growth curves of CECs in the static and perfusion systems after seeding at an initial cell number of 0.7 × 10⁴ cells/well.