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. 2017 Apr 10;7:777. doi: 10.1038/s41598-017-00914-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impediment of EMT in CECs by biomimetic platforms of CEC spheroids. (A–G) QPCR analysis of proliferation marker and functional marker expression normalized to GAPDH. (H) WB of vimentin, ATP1A1, AQP1 and GAPDH in 2D and SP cultures. (I) Quantification of protein expression levels by Western blotting using Image J software. Differences with *P < 0.05 were considered statistically significant. (J) Hierarchical cluster analysis of gene expression based on log ratio RPKM data. (K) Differences in the gene expression profiles of 2D and SP cultures.