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. 2017 Apr 7;7:729. doi: 10.1038/s41598-017-00855-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of LV dilatation and hypertrophy caused by severe aortic regurgitation on cardiomyocyte cross-sectional area (Top panels; A and B) and on myocardial capillary density (bottom panels (C and D). Left panels (A and C): The results are expressed as the mean ± SEM (n = 6–7/gr.). Probability values are from a 2-way ANOVA and Holm-Sedak post-testing. *p < 0.05 or **p < 0.01 between indicated groups. Upper right panels: Representative Masson’s trichrome staining of mid-chamber short-axis LV sections (magnification x200); bottom right panels: LV sections stained with isolectin B4 coupled with horseradish peroxidase (magnification x400).