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. 2017 Apr 11;7:812. doi: 10.1038/s41598-017-00845-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTEN activity modulates collagen expression in human TM cells. TM cells were treated with TGF-β2 (5 ng/ml) and transfected with control GFP-vector (Cv), enhanced PTEN-GFP vector (ePv) or C124SPTEN-GFP vector (C124SPv). Enhanced PTEN has eight-fold increased ability to suppress PIP3 signaling, while C124SPTEN has defective lipid phosphatase activity. Densitometry was performed on collagen Western blots using ImageJ software. Statistical significance was determined using one-way ANOVA with Tukey’s post-hoc. *p < 0.05, **p < 0.01, ****p < 0.0001. N = 3. D5975- representative human donor. Transfection with enhanced PTEN decreased the levels of TGF-β-induced collagen to that of controls, while reducing the levels of PTEN and its phosphorylation. Transfection with C124SPTEN enhanced TGF-β induced increase in collagen levels and this was accompanied by an increase in the levels of PTEN and phosphorylated PTEN.