Figure 4.
(A) Principles of the soft X-ray microscope, operated at 520 eV in the so-called “water window” range19. Soft X-ray projection images are obtained in one-degree consecutive steps for an angular range up to 131 degrees. (B) The images from all angles are combined to reconstruct a tomogram where different compartments of the infected red blood cell are segmented and given color for clarity. (C) X-ray fluorescence imaging. The sample is raster scanned in both dimensions perpendicular to the incident 17.05 keV beam, and the fluorescent X-rays are detected at the direction given by the angles u and α. The angle u was fixed at 17.8° whereas the angle between the incident X-ray beam and the normal to the grid plane, α, can be varied. With α > 20° the fluorescent rays are not intercepted by the Cu grid and will all enter the detector. (D) Fluorescent spectrum summed over all pixels defined by a mask. The large Cu signal is due to stray fluorescence from the Cu grid. The fit is derived by PyMCA program provided by ESRF.