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. 2017 Apr 11;7:802. doi: 10.1038/s41598-017-00921-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fe map of a pristine red blood cell (RBC). One pixel is 50 nm. The rectangles are masks of raster areas from which X-ray fluorescence spectra are derived. Within masks 1–4 the scanning X-ray beam has illuminated the volumes outside the RBC with a traversed thickness of about 19 μm of ice. In mask 5, according to the tomogram of this sample, a 5.4 μm thickness of the RBC plus 13.6 μm of ice upstream and downstream have been illuminated.