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. 2017 Apr 11;7:788. doi: 10.1038/s41598-017-00909-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Brefeldin A induced rapid Golgi fragmentation. HeLa cells were treated with 0.2 μM BFA for the indicated times, then fixed and stained for GM130 and the Draq5. (A) Intact, Partially and fully fragmented populations were analyzed by IFC at each time point. (B) Representative IFC images of cells from non-treated and 60 min BFA treatment. (C) HeLa cells were treated with 0.2 μM BFA or vehicle for 60 min or left untreated. Cells were fixed and stained for GM130 and DAPI. Cells were imaged by confocal spinning disc microscope, manually counted and classified into the 3 Golgi populations. More than 150 cells were counted for each treatment, n = 2. (D) HeLa cells were treated with 0.2 μM BFA for 60 min or left untreated. Representative histograms of PulSA values (the width of the GM130 signal) for GM130 of two independent experiments.