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. 2017 Apr 11;7:834. doi: 10.1038/s41598-017-00970-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Body weight and composition. Body weight and composition of mice at different ages (specified in the figure) were determined using magnetic resonance imaging approach as described in the method section. (a) Body weight, lean and fat mass to body weight ratio. (b) Body weight, lean and fat mass normalized to young mice with the same gender and genotype. Male: 5–8 m mice, n = 17 for WT, n = 13 for TSC1 ^tg; 20–22 m mice, n = 18 for WT, n = 10 for TSC1 ^tg. Female: 12–14 m mice, n = 14 for WT, n = 15 for TSC1 ^tg; 20–22 m mice, n = 12 for WT, n = 19 for TSC1 ^tg. The asterisks indicate statistical significance between Tsc1 ^tg and wild type mice (*p < 0.05).