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. 2017 Apr 11;7:834. doi: 10.1038/s41598-017-00970-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fibrosis and inflammatory response in the heart of aged mice and the heart of mice after isoproterenol challenge. (a) Western blot analysis of NFκB, Stat3, FGF2 and MMP2/9 in heart protein extracts from old female mice (22–24 months of age, n = 6). (b) Quantification of protein band intensity (n = 6). WT – wild type mice; TSC1 - Tsc1 ^tg mice. White bar – saline-injected mice; black bar – isoproterenol-injected mice. Asterisks (*p < 0.05 and **p < 0.01) indicate statistical significance between isoproterenol injected and saline injected mice of the same genotype as indicated in the graph.