Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 11;7:810. doi: 10.1038/s41598-017-00999-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

LPS does not induce IE gene expression in latently infected monocytes. (A) Monocytes were infected with Merlin and 3 dpi analysed for UL138 and UL123 expression by qRT-PCR and then calculated relative to GAPDH control. RNA without prior RT was analysed concurrently (no RT) n = 3. (B) Latently infected monocytes were either treated with media alone (mono), 50–5000 ng/ml LPS (Mono + LPS) or differentiated with IL-4/GM-CSF and then stimulated with 50 ng/ml LPS (iDC + LPS). Eight hours post stimulation, RNA was harvested and analysed for UL123 (MIE) gene expression and expressed relative to GAPDH. RNA without prior RT was analysed concurrently (no RT) n = 3. **p < 0.01; NS = non-significant difference when compared to monocyte control. (C) Latently infected monocytes were either treated with media alone (mono), 500 ng/ml LPS (Mono + LPS) or differentiated with IL-4/GM-CSF and then stimulated with 50 ng/ml LPS (iDC + LPS). Cells were then co-cultured with fibroblasts for 17 days which were stained for IE protein expression to identify infectious centres n = 3. **p < 0.01; NS = non-significant difference when compared to monocyte control.