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. 2017 Apr 12;7:839. doi: 10.1038/s41598-017-00876-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glutathione (GSH) metabolic network analysis using the dape module (KEGG map00480, reproduced with permission of Kanehisa Laboratories, Japan). Boxes represent reactions while small circles represent compounds. Core reactions are colored blue, variable reactions are colored orange; blue circles represent compounds better used by PhTAC125 at 4 °C, compared to PspTB41. The orange reactions indicates that PspTB41 is not able to carry out reactions catalyzed by glutathionylspermidine amidase/synthetase (3.5.1.78) and glutathionylspermidine synthase (6.3.1.8), resulting in its inability on producing glutathionylspermidine and S-thiolation. Gluathione metabolism is strictly related to ‘Arginine and Proline metabolism’.