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. 2017 Apr 12;7:845. doi: 10.1038/s41598-017-00995-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Bright-field image of a test sample containing bundles of peripheral nerve fibers (N), connective tissue (C), and a skeletal muscle tissue (M). The scale bar is 1 mm. (b) Bright-field image of the sample together with multiple excitation laser irradiation spots. (c) Raman spectra obtained at the laser spots seen below numbers in (b). The signal accumulation time was 5 seconds. N, C, and M indicate the tissue type at each spot position. The arrowheads indicate two characteristic bands at 2895 and 2931 cm⁻¹. The spectra were preprocessed.