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. 2017 Apr 12;7:845. doi: 10.1038/s41598-017-00995-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Visualizing the nerve bundles by morphology-highlighted images, the tissue type maps, and the nerve detection criterion on the N _N/N _total. (a–c) The overlay of morphology-highlighted images, and tissue type maps of samples shown in Fig. 3. At red, yellow, and green points, tissues are discriminated as peripheral nerve, connective, and skeletal muscle tissues, respectively. (d–f) The nerve bundles highlighted in red according to the nerve detection criterion of N _N /N _total = or >0.4.