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. 2017 Apr 12;7:858. doi: 10.1038/s41598-017-00980-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The HK CbrA is necessary for glucose utilization. (a) Growth kinetics of the reference strain AEalgD (algD::Km) (closed symbols) and its derivative mutant AH1 (cbrA::miniTn5) (open symbols) in liquid Burk’s minimal medium amended with 50 mM glucose (dashed lines) or 2% sucrose (solid lines). (b) Growth kinetics (circles), and acetate (triangles) or glucose (diamonds) consumption during the culture of the reference strain AEalgD (algD::Km) (closed symbols) or its derivative mutant AH1 (cbrA::miniTn5) (open symbols) in Burk’s minimal medium supplemented with both 30 mM glucose and 30 mM acetate as carbon sources. (c) gluP mRNA levels determined by qRT-PCR analysis in strain AEalgD (white columns) and in mutant AH1 (grey columns) in the diauxic growth of panel (b). Total RNA was extracted from cells at the indicated times. The bars of standard deviation from three independent experiments are shown. Significant differences were analysed by t-test. Statistical significance is indicated (***p < 0.001).