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. 2017 Apr 13;7:877. doi: 10.1038/s41598-017-00707-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity-dependent modulation of FOXP1 recruitment to CtBP1 co-repressor complexes. (A) Glutamate receptor activation inhibits FOXP1 binding to Ctbp1. Co-immunoprecipitation of Ctbp1 complex and FOXP1 following glutamate stimulation of cultured cortical neurons was preceded by a fixation step using PFA to prevent deSUMOylation followed by immunoblotting with FOXP1, Ctbp1, LSD1 and HDAC2 antibodies. (B) Quantification of FOXP1 binding as shown in (A) shows marked reduction in the Ctbp1-FOXP1 interaction. Student’s t-test, n = 3, *p < 0.05. (C) Histogram of LSD1 binding as shown in (A) shows no-change in the Ctbp1-LSD1 interaction. (D) Quantification of HDAC2 binding as shown in (A) shows a decrease in the Ctbp1-HDAC2 interaction. Student’s t-test, n = 3, *p < 0.05.