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. 2017 Apr 13;7:885. doi: 10.1038/s41598-017-01005-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterizing the coverage distribution with the Unevenness (U_E) score. (A) The coverage distribution from multiple samples is plotted against the exon length. (B) The smoothed median coverage plotted against the exon length, obtained by first calculating median coverage for each position and then using LOWESS smoothing. Peaks and troughs were then identified by using a local optimization algorithm. Arrows indicate peaks identified in the curve: B, base, W, width and H, height of the peak, L_R, length of the region analyzed.