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. 2017 Apr 13;7:874. doi: 10.1038/s41598-017-00923-0

Figure 2.

Figure 2

Effect of AdMSC on cytokine production in cocultures of PBMC stimulated with anti-CD3 antibody. Production of cytokines TNF-α, IFN-γ and IL-10 (pg/ml) in the supernatant of cocultures of AdMSC + PBMC at AdMSC: PBMC ratio (1:10, 1:50 and 1:100) after 1, 3 and 5 days of culture, measured by kit “Human Th1/Th2 CBA” (Cytometric bead Array) and analyzed by flow cytometry (n = 9 experiments with AdMSC derived from different individuals). AdMSC/PBMC interactions decreased TNF-α at all time points; induced an increase of IL-10 at day 1, and a decreased of IFN γ at days 3 and 5 (A). Detection limits of 5 pg/ml 5000 pg/ml. Mann Whitney test *p < 0.05, **p < 0.001. Only in assays with highly suppressive AdMSC we observed a positive correlation between the inhibition of TNF-α, and IFN-γ at day one of coculture (Correlation coefficient, ρ = 1.000, p = 0.0000) (B). For the assays displaying low suppression, the positive correlation between IFN-γ and TNF-α decrease appeared later, at days 3 (ρ = 0.8627, p = 0.0270) and 5 (ρ = 0.9963, p = 0.0000) (data no shown).