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. 2017 Apr 18;7:903. doi: 10.1038/s41598-017-01017-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bactericidal Effect of Ag Potentiated by Transition Metals. Log change in CFUs/mL with respect to time zero, in Escherichia coli ATCC 11229 after 1 hour treatment with: LB (control), Ag, specific transition metal and their combination. As nominal concentrations: (A) Ag 30 µM, Ni 0.5 mM and the combination; (B) Ag 30 µM, Cd 1 mM and the combination; (C) Ag 15 µM, Cu 1 mM and 2 mM, and the respective combinations; (D) Ag 30 µM, Zn 0.5 mM and the combination. ***Corresponds to a p < 0.05, tested with an ANOVA, that there is a difference with respect to the control and each of the individual treatments. Error bars correspond to the standard deviation from experiments performed in triplicates.