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. 2017 Apr 18;7:903. doi: 10.1038/s41598-017-01017-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytotoxicity effects of Ag and transition metals on H9c2 and B16F10 cells. (A) H9c2 and B16F10 cells were treated with Ag at the indicated concentrations for 24 h and viability was determined. (B) Effect of transition metals on the viability of cells H9c2. Cells were treated with each transition metal at a concentration of 1 mM for 48 h and viability was determined. Bars represent means of three independent experiments and their respective standard deviations. (C) Dose-response effect of transition metals on the viability of H9c2 cells. Cells were treated with transition metals at the stated concentration for 48 h before viability was determined. Indicator points represent means of three independent experiments and their respective standard deviations. Values are mean ± SD (n = 3 experiments at least for each treatment).