Table 2.

Comparison of Hes-1, OPN concentrations in atherosclerotic plaques and normal arterial intimal tissues using Immunohistochemistry, Immunofluorescence and Image Analysis.

	Immunohistochemistry			Immunofluorescence
Protein	normal tissues	atherosclerotic plaques	p	normal tissues	atherosclerotic plaques	p
Hes-1	54.89 ± 19.73	4.26 ± 2.14	<0.05	99.95 ± 19.32	9.42 ± 10.15	<0.05
OPN	9.79 ± 7.46	96.75 ± 22.78	<0.05	4.00 ± 3.40	144.1 ± 13.97	<0.05

Figure presented are mean optical densities minus background staining from negative slides ± standard error. Hes-1, hairy/enhancer of split homologue-1; OPN indicates osteopontin.