Table 1.
Absorption at different wavelengths of C. merolae crude extract and the different fraction of ammonium sulfate treated extract
| Crude extract | Supernatant fraction of 20% (NH4)2SO4 | Supernatant fraction of 40% (NH4)2SO4 | Pellet fraction of 40% (NH4)2SO4 | Supernatant fraction of 60% (NH4)2SO4 | Pellet fraction of 60% (NH4)2SO4 | |
|---|---|---|---|---|---|---|
| Volume of sample (mL) | 40 | 40 | 40 | 10 | 40 | 10 |
| Dilution | 1:5 | 1:5 | 1:5 | 1:20 | 1:5 | 1:20 |
| A652 | 0.098 | 0.096 | 0.032 | 0.059 | 0.019 | 0.031 |
| A624 | 0.496 | 0.469 | 0.167 | 0.271 | 0.042 | 0.147 |
| A562 | 0.145 | 0.135 | 0.049 | 0.135 | 0.017 | 0.048 |
| A280 | 0.05 | 0.051 | 0.025 | 0.015 | 0.073 | 0.014 |
| Purity index | 9.92 | 9.26 | 6.78 | 18.07 | 0.58 | 10.78 |
| Total C-PC | 16.8 | 15.8 | 5.6 | 9.2 | 1.2 | 5 |
| Yield | 100 | 94.31 | 33.81 | 54.06 | 7.38 | 31.32 |