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. 2017 Apr 30;2017:7898973. doi: 10.1155/2017/7898973

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Copyright © 2017 Atsuyoshi Nishina et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of 1 or 9 with or without Akt or ERK5 inhibitor on inflammatory rerated proteins in RAW 264.7 cells. RAW 264.7 cells were seeded into a 6-well plate (3 × 10⁵ cells/well) and incubated for 2 hours. Then they were treated with an inhibitor of appropriate concentration for one hour followed by stimulation with LPS of 100 ng/well with or without various concentration of a dimethyl sulfoxide (DMSO) solution of a 5,7-dihydroxyflavone analogue for 15 min (IκBα, Akt, and Erk5) or 16 hours (iNOS and COX2). Labels present the compound names and concentrations (μM). Data are expressed as mean ± SD from 3 independent experiments. Control group were presented by the open circles. ^∗Significance: p < 0.05 and ^∗∗Significance: p < 0.01.