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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Metab Eng. 2016 Nov 14;43(Pt B):198–207. doi: 10.1016/j.ymben.2016.11.004

Figure 6. Altered anaplerotic contributions in CTP-deficient cells.

Figure 6

A) Selected malate and citrate MIDs in cells cultured in [U-13C]glutamine.

B) Selected malate and citrate MIDs in cells cultured in [U-13C]glucose.

C) Selected malate and citrate MIDs in cells cultured in [3,4-13C]glucose to assess flow through pyruvate carboxylase (PC).

D) Western-blot of PC expression in CTP-WT and CTP-KO cells.

Data are displayed as mean ± S.D. *, p<0.05, two-way ANOVA followed by Sidak’s multiple comparisons test. n=3 cultures of a representative experiment. All experiments were repeated 3 times. Two independent pools of CTP-KO clones (pools KO1 and KO2) were analyzed for all experiments.