Figure 6. TRPV4 −/− mice have impaired BK channel activity in the CD.

(A) Representative current traces of single channel BK activity in CD cells from WT (top) and TRPV4−/− mice (bottom) kept on high (5% K⁺) potassium diet for 1 week. The patches were held at a test potential of V_h=−V_p=+100 mV. Outward K⁺ currents are upward. Dashed lines indicate the respective current state with c denoting the closed state. (B) Pie charts representing the frequency of observing patches with active channels (f) for the conditions described in (A). (C) Summary graph of functional BK levels (fN) for WT and TRPV4 −/− mice kept on high potassium diet. * – significant decrease vs WT.