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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Tissue Res. 2017 Feb 8;368(3):615–627. doi: 10.1007/s00441-017-2575-2

Fig. 3. Nephrocyte uptake of hemolymph protein.

Fig. 3

a Second instar larvae nephrocytes in which the indicated Rab gene was silenced were quantitatively assessed for uptake of ANF-RFP fusion protein from the hemolymph. Red fluorescent protein (RFP) fused to rat atrium natriuretic factor (ANF) is expressed in and secreted by muscle cells from a transgene driven by the MHC enhancer. ANF-RFP fluorescence in nephrocytes expressed as percent of WT control. b Fluorescence microscopy showing ANF-RFP uptake (red) by nephrocytes (GFP nuclear expression) in which the indicated Rab gene expression was silenced. Dotted lines indicate outlines of nephrocytes. c Adult flies in which the indicated Rab gene was silenced were quantitatively assessed for uptake of ANF-RFP fusion protein from the hemolymph. ANF-RFP in nephrocytes expressed as percent of WT control. d Fluorescence microscopy showing ANF-RFP (red) uptake by nephrocytes (GFP nuclear expression) in which the indicated Rab gene expression was silenced. Asterisks indicate missing nephrocytes as a result of Rab1 and Rab5 silencing. For quantification, 20 nephrocytes were analyzed from each of 3 larvae or flies per genotype. The results are presented as mean±s.d. Results were analyzed by Student’s t-test. Statistical significance was defined as P<0.05.

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