Fig. 4. AgNO3 Uptake and Developmental Toxicity Assays.
a Third instar larvae nephrocytes in which the indicated Rab gene was silenced were quantitatively assessed for sequestration of ingested AgNO3. AgNO3 in nephrocytes expressed as percent of WT control. b Micrographs showing sequestration of ingested AgNO3 in larval pericardial nephrocytes in which the indicated Rab gene expression was silenced. Dotted lines indicate outlines of nephrocytes. c Adult nephrocytes in which the indicated Rab gene was silenced were quantitatively assessed for sequestration of ingested AgNO3. AgNO3 in nephrocytes expressed as percent of WT control (note that Rab1 and Rab5 silencing result in absence of adult nephrocytes). d Micrographs showing sequestration of ingested AgNO3 in adult pericardial nephrocytes in which the indicated Rab gene expression was silenced. Dotted lines indicate outlines of nephrocytes. e Developmental toxicity from ingestion of dietary AgNO3. Mortality index is expressed as the % of second instar larvae of the indicated Rab silencing genotype that fail to develop into adult flies. For quantification, 20 nephrocytes were analyzed from each of 3 larvae or flies per genotype. The results are presented as mean±s.d. Results were analyzed by Student’s t-test. Statistical significance was defined as P<0.05.