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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Osteoarthritis Cartilage. 2016 Nov 14;25(6):914–925. doi: 10.1016/j.joca.2016.11.004

Figure 4. Effect of blockade of CCR2 on bone after DMM-induced OA measured at the early, moderate and severe stage.

Figure 4

Bone sclerosis induced by DMM (BV/TV) was measured by μ-CT at the medial tibia plateau (A) 4wks, (B) 8wks and (C) 12wks after DMM/sham, treated with (N=3, for both Sham and DMM at each of the time points analyzed) and without RS-504393 (N=5, for both Sham and DMM at each of the time points analyzed) at the times indicated in Fig. 2A (A, 1-to-4wks; B, 4-to-8wks; C, 8-to-12wks). Representative mid-coronal sections are presented with a segmentation mask equivalent to that applied during quantitative bone morphometry analysis. (D) Osteophyte size scoring was measured from sections (4/mouse) obtained from the anterior /medial margin of the tibia from DMM-operated knees at 8 weeks (N= 6 for both untreated Sham and DMM; for RS-504393 treated samples, N= 5 for Sham and N=6 for DMM) and 12 weeks (for untreated samples, N=5 for Sham and N=4 for DMM; for RS-504393 treated samples, N= 6 for both Sham and DMM) after surgery, with or without RS-504393 treatment at the time indicated in Fig. 2A (4-to-8wks or 8-to-12wks). All graphs represent the mean ± standard deviation. Significance was set at P < 0.05.