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. 2017 May 15;8:872. doi: 10.3389/fmicb.2017.00872

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Copyright © 2017 Hajam and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo safety evaluation of the vaccine. Mice were either inoculated intramuscularly with the wild type strain JOL990 (Sal-990, n = 8), PBS only, JOL1837 carrying empty vector (Sal-vector) and constructs JOL1917+1913 (im-Sal-HA-M2e) or intraperitoneally with constructs JOL1913+1917 (ip-Sal-HA-M2e) or orally with constructs JOL1913+1917 (po-Sal-HA-M2e). The animals were monitored for 28 days and the mortality of the animals was recorded. Non-lethal endpoints were used during the experiments and mice were euthanized to limit suffering, when they exhibited weight loss of more than 30%, lethargy, ruffled hair coat, or hunched posture.