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. 2017 May 15;8:872. doi: 10.3389/fmicb.2017.00872

FIGURE 4.

FIGURE 4

Efficient humoral immune responses are stimulated in BALB/c mice (n = 6) after vaccination with JOL1913+1917. Mice were either immunized intramuscularly with the PBS only, JOL1837 carrying the empty vector pJHL65 (Sal-vector) and constructs JOL1913+JOL1917 (im-Sal-HA-M2e), or intraperitoneally with JOL1913+JOL1917 (ip-Sal-HA-M2e) or orally with constructs JOL1913+1917 (po-Sal-HA-M2e). The antibodies IgG1 and IgG2a were measured in serum at different time points post-vaccination by indirect ELISA. (I,II) Kinetics of HA-specific IgG1 and IgG2a responses, respectively, in sera from vaccinated mice. (III,IV) Kinetics of M2e-specific IgG1 and IgG2a responses, respectively, in sera from vaccinated mice. The assays are performed in duplicate and the data are presented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01; asignificant with respect to PBS, Sal-vector and po-Sal-HA-M2e groups; bsignificant with respect to Sal-vector and PBS controls; dpi, days post-immunization; ns, non-significant.