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. 2017 May 15;8:295. doi: 10.3389/fphys.2017.00295

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Copyright © 2017 Keshavarz, Schwarz, Hartmann, Wiegand, Skill, Althaus, Kummer and Krasteva-Christ.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of cav-1 in murine thoracic organs using immunohistochemistry. Images are representative for cav-1^+/+ (n = 3 animals) and cav-1^−/− mice (n = 4 animals). (A–C) Cav-1-immunoreactivity is observed in endothelial cells of the esophagus and trachea and in tracheal basal epithelium and both tracheal and bronchial SM. (D,E) Alveolar region of the lung, bronchial SM and cells present in the alveolar wall (endothelial cells, epithelial cells) are immunoreactive for cav-1. (F) Aorta. (G–I) Controls for the specificity of the anti-cav-1 antibody. The cav-1-labeling is not present in cav-1^−/− trachea, lung and aorta. Arrowhead, endothelial cell; double arrowhead, basal epithelium; arrow, SM; lu, lumen; es, esophagus; bar, 50 μm.